Research article summary (published 25 Jun 2003):

A targeting motif involved in sodium channel clustering at the axonal initial segment.

Full Abstract

The sorting of sodium channels to axons and the formation of clusters are of primary importance for neuronal electrogenesis. Here, we showed that the cytoplasmic loop connecting domains II and III of the Nav1 subunit contains a determinant conferring compartmentalization in the axonal initial segment of rat hippocampal neurons. Expression of a soluble Nav1.2II-III linker protein led to the disorganization of endogenous sodium channels. The motif was sufficient to redirect a somatodendritic potassium channel to the axonal initial segment, a process involving association with ankyrin G. Thus, this motif may play a fundamental role in controlling electrical excitability during development and plasticity.

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Author information

Author/s: Garrido, Juan José (JJ); Giraud, Pierre (P); Carlier, Edmond (E); Fernandes, Fanny (F); Moussif, Anissa (A); Fache, Marie-Pierre (MP); Debanne, Dominique (D); Dargent, Bénédicte (B);

Affiliation: Institut National de la Santé et de la Recherche Médicale Unité 464, Institut Jean Roche, Université de la Méditerranée, Faculté de Médecine Secteur-Nord, Boulevard P. Dramard, 13916 Marseille Cedex 20, France.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Science (New York, N.Y.) (Science), published in United States. (Language: eng)

Reference: 2003-Jun; vol 300 (issue 5628) : pp 2091-4

Dates: Created 2003/06/27; Completed 2003/07/11; Revised 2007/03/19;

PMID: 12829783, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Motifs Amino Acid Sequence Animals Ankyrins - metabolism Axons - metabolism Cell Membrane - metabolism Delayed Rectifier Potassium Channels Hippocampus - cytology Humans Ion Channel Gating Molecular Sequence Data Mutation

Mutation Nerve Tissue Proteins - chemistry; genetics; metabolism Neurons - metabolism Patch-Clamp Techniques Potassium Channels - metabolism Potassium Channels, Voltage-Gated Protein Structure, Tertiary Protein Transport Rats Recombinant Fusion Proteins - chemistry; metabolism Sodium Channels - chemistry; genetics; metabolism Transfection

Associated Chemicals: Ankyrins (0) ; Delayed Rectifier Potassium Channels (0) ; Nerve Tissue Proteins (0) ; Potassium Channels (0) ; Potassium Channels, Voltage-Gated (0) ; Recombinant Fusion Proteins (0) ; Sodium Channels (0) ; sodium channel, voltage-gated, type II, alpha 1 (0)

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