A structure-function analysis of glial cell-line-derived neurotrophic factor receptor alpha1.

Full Abstract

The GFRalpha1 cDNA was amplified by RT-PCR from fetal rat hippocampus. The soluble recombinant GFRalpha1 and its mutants were obtained from an Escherichia coli expression system. The biological activity of soluble GFRalpha1 and its mutants were evaluated in PC12 cells. The results suggest that the central domain of GFRalpha1 is a crucial determinant for ligand binding. This established a solid basis for further study to find the key amino acid mediating the binding of GDNF and GFRalpha1.

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Author information

Author/s: Wang, Li-Mei (LM); Chen, Zhe-Yu (ZY); Zhang, Qing (Q); Zhu, Wei (W); Ding, Da-Fu (DF); Lu, Chang-Lin (CL); He, Cheng (C);

Affiliation: Department of Neurobiology, the Second Military Medical University, 800 Xiangyin Road, Shanghai, 200433, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Protein and peptide letters (Protein Pept Lett), published in Netherlands. (Language: eng)

Reference: 2003-Feb; vol 10 (issue 1) : pp 61-72

Dates: Created 2003/03/10; Completed 2003/08/29; Revised 2006/11/15;

PMID: 12625827, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence
Animals
Cell Differentiation - drug effects
Cell Survival - drug effects
DNA Primers - genetics
Escherichia coli - metabolism
Gene Deletion
Glial Cell Line-Derived Neurotrophic Factor Receptors
Hippocampus - chemistry
Humans
Models, Molecular

Models, Molecular
PC12 Cells
Protein Binding
Protein Structure, Secondary
Protein Structure, Tertiary - genetics
Proto-Oncogene Proteins - genetics; metabolism; pharmacology
Proto-Oncogene Proteins c-ret
Rats
Receptor Protein-Tyrosine Kinases - genetics; metabolism; pharmacology
Recombinant Proteins - chemistry; genetics; metabolism; pharmacology
Structure-Activity Relationship

Associated Chemicals: DNA Primers (0) ; GFRA1 protein, human (0) ; Gfra1 protein, rat (0) ; Glial Cell Line-Derived Neurotrophic Factor Receptors (0) ; Proto-Oncogene Proteins (0) ; Recombinant Proteins (0) ; Proto-Oncogene Proteins c-ret (EC 2.7.1.112) ; Receptor Protein-Tyrosine Kinases (EC 2.7.1.112)

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