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| Research article summary (published 29 Apr 2003): |
Simulations of voltage clamping poorly space-clamped voltage-dependent conductances in a uniform cylindrical neurite.
Full Abstract
Significant error is made by using a point voltage clamp to measure active ionic current properties in poorly space-clamped cells. This can even occur when there are no obvious signs of poor spatial control. We evaluated this error for experiments that employ an isochronal I(V) approach to analyzing clamp currents. Simulated voltage clamp experiments were run on a model neuron having a uniform distribution of a single voltage-gated inactivating ionic current channel along an elongate, but electrotonically compact, process. Isochronal Boltzmann I(V) and kinetic parameter values obtained by fitting the Hodgkin-Huxley equations to the clamp currents were compared with the values originally set in the model. Good fits were obtained for both inward and outward currents for moderate channel densities. Most parameter errors increased with conductance density. The activation rate parameters were more sensitive to poor space clamp than the I(V) parameters. Large errors can occur despite "normal"-looking clamp curves.
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Author information
Author/s: Hartline, Daniel K (DK); Castelfranco, Ann M (AM);
Affiliation: Békésy Laboratory of Neurobiology, Pacific Biomedical Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Journal: Journal of computational neuroscience (J Comput Neurosci), published in United States. (Language: eng)
Reference: -2003 May-Jun; vol 14 (issue 3) : pp 253-69
Dates: Created 2003/05/26; Completed 2003/07/11; Revised 2006/11/15;
PMID: 12766427, status: MEDLINE (last retrieval date: 11/6/2008)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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