Research article summary (published 26 Jul 2003):

Infectious HIV-1 assembles in late endosomes in primary macrophages.

Full Abstract

Although human immunodeficiency virus type 1 (HIV-1) is generally thought to assemble at the plasma membrane of infected cells, virions have been observed in intracellular compartments in macrophages. Here, we investigated virus assembly in HIV-1-infected primary human monocyte-derived macrophages (MDM). Electron microscopy of cryosections showed virus particles, identified by their morphology and positive labeling with antibodies to the viral p17, p24, and envelope proteins, in intracellular vacuoles. Immunolabeling demonstrated that these compartments contained the late endosomal marker CD63, which was enriched on vesicles within these structures and incorporated into the envelope of budding virions. The virus-containing vacuoles were also labeled with antibodies against LAMP-1, CD81, and CD82, which were also incorporated into the viral envelope. To assess the cellular source of infectious viruses derived from MDM, virus-containing media from infected cells were precipitated with specific antibodies. Only antibodies against antigens found in late endosomes precipitated infectious virus, whereas antibodies against proteins located primarily on the cell surface did not. Our data indicate that most of the infectious HIV produced by primary macrophages is assembled on late endocytic membranes and acquires antigens characteristic of this compartment. This notion has significant implications for understanding the biology of HIV and its cell-cell transmission.

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Author information

Author/s: Pelchen-Matthews, Annegret (A); Kramer, Beatrice (B); Marsh, Mark (M);

Affiliation: Cell Biology Unit, Medical Research (MRC) Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Journal of cell biology (J Cell Biol), published in United States. (Language: eng)

Reference: 2003-Aug; vol 162 (issue 3) : pp 443-55

Dates: Created 2003/08/05; Completed 2003/09/12; Revised 2007/11/15;

PMID: 12885763, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Antigens, CD - metabolism Cell Compartmentation - physiology Endosomes - metabolism; ultrastructure; virology Gene Products, gag - metabolism HIV Antigens - metabolism HIV-1 - metabolism; pathogenicity; ultrastructure Humans Immunohistochemistry Intracellular Membranes - metabolism; ultrastructure; virology Kangai-1 Protein Lysosome-Associated Membrane Glycoproteins Macrophages - metabolism; ultrastructure; virology Membrane Glycoproteins - metabolism

Membrane Glycoproteins - metabolism Membrane Proteins - metabolism Microscopy, Electron Monocytes - metabolism; ultrastructure; virology Proto-Oncogene Proteins Reaction Time - physiology Retroviridae Proteins, Oncogenic - metabolism Vacuoles - metabolism; ultrastructure; virology Viral Envelope Proteins - metabolism Viral Proteins Virus Replication - physiology Virus Shedding - physiology gag Gene Products, Human Immunodeficiency Virus

Associated Chemicals: Antigens, CD (0) ; CD81 antigen (0) ; CD82 protein, human (0) ; Gene Products, gag (0) ; HIV Antigens (0) ; Kangai-1 Protein (0) ; Lysosome-Associated Membrane Glycoproteins (0) ; Membrane Glycoproteins (0) ; Membrane Proteins (0) ; Proto-Oncogene Proteins (0) ; Retroviridae Proteins, Oncogenic (0) ; Viral Envelope Proteins (0) ; Viral Proteins (0) ; gag Gene Products, Human Immunodeficiency Virus (0) ; p17 protein, Human Immunodeficiency Virus Type 1 (0) ; p24 protein, Human T-lymphotropic virus 1 (0)

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