Cloning and characterization of a novel human 5-HT4 receptor variant that lacks the alternatively spliced carboxy terminal exon. RT-PCR distribution in human brain and periphery of multiple 5-HT4 receptor variants.

Full Abstract

We have cloned a novel C-terminal splice variant of serotonin 5-HT4 receptors from human hippocampus. The deduced protein extends only one aminoacid past the splicing point. We propose to call the novel variant h5-HT4(n) since it contains none of the C-terminal exons alternatively spliced in other variants. The pharmacological profile of h5-HT4(n) stably expressed in HeLa cells is in agreement with other reported variants. Stably transfected cells showed increased basal levels of intracellular cAMP in absence of agonist, indicating constitutive activity of the expressed receptors. 5-HT induced robust increases of intracellular cAMP. The 5-HT4 receptor antagonist GR 113808 blocked the effects of 5-HT and brought intracellular cAMP below basal constitutive levels, indicating inverse agonism of this compound in this system. The RT-PCR distribution of all known human C-terminal splice variants in human brain regions and periphery showed complex patterns of variant expression, with the novel variant h5-HT4(n) being widely and abundantly expressed.

Learn Faster Today Improve your study skills

Author information

Author/s: Vilaró, M T (MT); Doménech, T (T); Palacios, J M (JM); Mengod, G (G);

Affiliation: Department of Neurochemistry, Instituto Investigaciones Biomédicas de Barcelona, CSIC-IDIBAPS, c/Rosselló 161, 6a, 08036, Barcelona, Spain.

Journal and publication information

Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't

Journal: Neuropharmacology (Neuropharmacology), published in England. (Language: eng)

Reference: 2002-Jan; vol 42 (issue 1) : pp 60-73

Dates: Created 2001/12/25; Completed 2002/02/20; Revised 2006/11/15;

PMID: 11750916, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alternative Splicing - genetics
Animals
Brain Chemistry - genetics
Cell Line
Cell Membrane - metabolism
Cloning, Molecular
Cyclic AMP - biosynthesis
DNA, Complementary - genetics
Exons - genetics
Female
Hippocampus - metabolism

Hippocampus - metabolism
Humans
Macaca fascicularis
Male
Oligonucleotides, Antisense - pharmacology
Peripheral Nervous System - metabolism
RNA, Messenger - biosynthesis; genetics
Radioligand Assay
Receptors, Serotonin - drug effects; genetics; metabolism
Receptors, Serotonin, 5-HT4
Reverse Transcriptase Polymerase Chain Reaction

Associated Chemicals: DNA, Complementary (0) ; Oligonucleotides, Antisense (0) ; RNA, Messenger (0) ; Receptors, Serotonin (0) ; Receptors, Serotonin, 5-HT4 (158165-40-3) ; Cyclic AMP (60-92-4)

These are the highest related articles currently in the database:

See 100+ related articles.